Sunday, April 22, 2012
What knowledge have you connected with the past knowledge?
The past knowledge I have connected with biochemistry is the DNA replication. I was taught about this replication in Biology and genetics which made it easier to connect more with it in my biochemistry class. DNA replication is a process that occurs in all living organisms and copies their DNA in a basis of biological inheritance. The DNA replication starts as a double stranded DNA structure that are coiled together and form a double helix. The structure that forms into a nucleus during DNA replication is called a replication fork, it is created by helicases which breaks down the hydrogen bonds holding the DNA strands together. DNA replication always starts from 5prime to 3prime direction. There are differences in the DNA replication in Prokaryotes and Eukaryotes. Prokaryotes is a well known mechanism such as DNA polymerases while Eukaryotes are more complex and still elucidating components of mechanism. In Prokaryotes polymerase are exonucleases while in Eukaryotes not all polymerases are exonucleases. There are one origin of replication in Prokaryotes while there are several origins in Eukaryotes. In prokaryotes there are 1000 to 2000 residues long in Okazaki fragments while in Eukaryotes there is only 150 to 200.
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